METABOLISM OF THYMINE (METHYL-C14 OR −2-C14) BY RAT LIVER IN VITRO

Studies on the metabolism of thymine-2-C14 by the rat.

It has been clearly established (1, 2), through the use of N16-labeled material, that the utilization of free thymine for the synthesis of deoxyribonucleic acid (DNA) by the rat is extremely limited. However, these studies afforded but little information concerning the rate and extent of the metabolism of thymine. In the experiments reported here, thymine-204 has been found to be more rapidly a...

Metabolism of pyruvic acid-2-C14 and hydroxypyruvic acid-2-C14 in algae.

The metabolism of pyruvic acid in the light and dark by Scenedesmus has been examined with two aims: to study the relationship of its metabolism to that of the intermediates of C1402 fixation and to compare its metabolic fate with that of hydroxypyruvate. The metabolism of hydroxypyruvic acid was compared with that of C1402 fixation in order to ascertain whether it might be a photosynthetic int...

Metabolism of Coprostanol-c14 and Cholestanol-4-c14 in Man.

The metabolism of ascorbic acid-1-C14 and oxalic acid-C14 in the rat.

It is known that oxalic acid is a product of ascorbic acid metabolism in the guinea pig (1) and rat (2), and is formed in man from unidentified precursors (3). Oxalates are also of interest because of their occasional toxicity and unexplained deposition. Ascorbic, dehydroascorbic, and 2,3diketogulonic acids are known to give rise to oxalic and threonic acids in approximately quantitative yields...

Metabolism of C14 labeled glucose and pyruvate by rat diaphragm muscle in vitro.

The results of a number of earlier investigations (l-5) have shown that glucose utilization and glycogen synthesis in the isolated rat diaphragm are increased by the addition of insulin to the incubation medium. Insulin also increases the amount of glucose carbon metabolized by mammalian muscle to CO*, both in vitro (5) and in vivo (6). The rates of these three reactions are increased by the re...